FastQCFastQC Report
Tue 31 May 2022
EGAF00005394340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160868071
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24458031.520378148874552Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5158080.320640383634612Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3386360.210505414713402No Hit
GCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCAC2065970.12842635503474148No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1898850.11803771800060935Clontech SMART CDS Primer II A (100% over 25bp)
GGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACG1828820.11368446134969816No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT1685700.10478773006484302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)