FastQCFastQC Report
Tue 14 May 2024
EGAF00005394367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102036565
Total Bases9.2 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21135002.0713162972508923Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4105320.4023381226132024Clontech SMART CDS Primer II A (100% over 24bp)
GCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCAC2005770.19657364984797362No Hit
GGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACG1948870.1909972175170734No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC1469940.1440601219768619No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG1354900.13278573225196283No Hit
CTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTT1307060.1280972169143483No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT1272040.12466511392264137No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC1222390.119799211194536No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT1121780.10993902038940648No Hit
GGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGT1062000.10408033629905122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)