FastQCFastQC Report
Sat 25 May 2024
EGAF00005394376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129231103
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22899171.771955006837634Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6409240.49595181432445096No Hit
GCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCAC2456580.19009200904212667No Hit
GGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACG2143980.16590278580226928No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT1703270.13180031435621192No Hit
CTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTT1688700.1306728767918974No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC1607470.12438723826415071No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC1485240.11492898888280788No Hit
GGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTG1408880.1090201946198664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)