FastQCFastQC Report
Tue 31 May 2022
EGAF00005394472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155411674
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58575653.7690637062438435Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12903520.830279969830323Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4847150.31189098445719077Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3077750.19803853345019629Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2907450.18708054068061836Clontech SMART CDS Primer II A (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2706380.17414264516576794No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580650.1660525193236127Clontech SMART CDS Primer II A (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)