FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16349328
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259620.15879551746713993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA308150.076.129279
CGGGTTA212100.075.5998469
GAGGTTA188100.072.796679
AGGGTTA189950.071.1703649
GGTTCCG145600.071.0158849
GGGTATA108600.069.5787669
TGGGTTA151750.069.56619
CCGGTTA176250.069.299879
GCACGTT106900.069.1745459
GGACGTT139600.068.557949
TTTTCCG130650.068.3751149
CGTTCCG96050.067.077469
CAGGTTA146650.067.029869
GGCATTA91200.066.683689
GTGGTTA167650.065.782239
TCACGTT94750.065.620559
CATTCCG72300.065.188069
TATTCCG99550.065.060669
CTGGTTA111550.065.0337459
GCGGTTA253300.064.947559
Produced by FastQC (version 0.11.5)