FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14484366
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222890.15388315926289076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA271000.076.246439
CGGGTTA188650.076.031029
GAGGTTA164100.072.566039
AGGGTTA176200.072.165499
TGGGTTA136950.071.218789
GGTTCCG129000.069.677829
GGACGTT126250.069.041179
CCGGTTA154550.068.360619
TTTTCCG113050.068.26839
CGTTCCG85750.068.0967569
GGCATTA83200.067.578789
GGGTATA93100.067.331189
GCACGTT95650.067.18019
ACGGTTA136800.067.165069
CAGGTTA134900.066.756399
CTGGTTA95600.065.659339
CATTCCG65400.065.112429
GTGGTTA149550.065.104739
TCACGTT80350.065.003969
CGACGTT90750.064.906849
Produced by FastQC (version 0.11.5)