FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12060484
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG191610.15887422096824638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA217600.076.6381159
CGGGTTA159350.075.049329
GAGGTTA133400.073.273469
AGGGTTA142300.071.557749
GGTTCCG105100.070.399699
TGGGTTA107350.069.953469
TTTTCCG96650.069.475229
GGGTATA77250.069.2080849
CGTTCCG66750.069.143799
CCGGTTA125000.068.9837349
GGACGTT107300.068.7582249
GGCATTA66950.067.667699
GCACGTT75950.066.755559
ACGGTTA114450.066.4307569
CATTCCG52650.065.7860649
CGACGTT74600.065.6848459
CTGGTTA79100.065.225429
CAGGTTA105200.065.1618359
GTGGTTA120700.064.997869
TCACGTT67200.064.823039
Produced by FastQC (version 0.11.5)