FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25152799
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG337520.13418784923300187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCCG214650.069.296949
GGCATTA138900.067.6493159
TTTTCCG186400.066.666619
CGTTCCG143650.066.654969
GGGTATA132550.065.72819
TGTTCCG182100.063.3631639
GGACGTT174300.060.956449
TATTCCG156900.060.1863569
CATTCCG97750.059.824449
GCACGTT133700.059.6318639
CGACGTT127050.058.4379779
AGTTCCG151150.058.1459359
GTACGTT132000.056.1499639
GATTCCG166650.055.543029
CGCATTA64100.055.4937869
TGGTATA114600.054.922139
GGTCAAT71900.054.0840459
TCACGTT107900.053.9211279
TTTCTCA89050.052.9743929
TAACGTT135950.052.8304639
Produced by FastQC (version 0.11.5)