FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25379858
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC687350.021.418710
CTGGCGA693250.021.2443479
TCTGGCG713400.021.1993798
GTCTGGC677850.021.1785897
ATGAGGG710700.021.023391
GGCGACC159750.020.89791311
GAGGGTC690050.020.8895853
GGCGACG170550.020.6258511
GGTCTGG700700.020.515126
TATGCCC127250.020.506061
TACGCCC102450.020.2924710
GGTGATC161900.020.2137373
AGGGTCT710850.020.0926424
GAAGATG128450.020.0905116
GTACGCC105000.019.8310819
CCATATG39450.019.7969447
CGGCTTG160400.019.7639949
CCCGATA60100.019.6221072
TGGACGC122000.019.5940951
TGAGGGT736250.019.542252
Produced by FastQC (version 0.11.5)