FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19797624
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC534250.021.44969610
CTGGCGA538750.021.2827849
TCTGGCG554750.021.2400048
GTCTGGC528800.021.2256167
ATGAGGG558500.020.9978371
GAGGGTC537200.020.9536843
GGCGACC124550.020.93080511
GGTCTGG543500.020.635866
GGCGACG134900.020.62140311
TATGCCC103050.020.603921
AGGGTCT553000.020.1720034
GGTGATC125850.020.1033293
GAAGATG98400.020.0883966
TACGCCC80050.019.9520110
AGAAACC70650.019.6484588
TGAGGGT572250.019.647132
GTACGCC81350.019.6331679
TGAAGAT95950.019.546625
CGGCTTG129100.019.5029749
GGCTTGG129100.019.46889310
Produced by FastQC (version 0.11.5)