FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19880621
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC542700.021.47680910
CTGGCGA546650.021.3417439
TCTGGCG561350.021.2864728
GTCTGGC536900.021.2560377
ATGAGGG563950.021.097881
GGCGACC129750.020.89763611
GAGGGTC548500.020.8752673
TATGCCC102750.020.759111
GGCGACG133800.020.63503511
GGTCTGG554700.020.5859976
TACGCCC82450.020.33208510
GGTGATC127850.020.262353
GAAGATG96150.020.2495276
AGGGTCT561200.020.2205664
GTACGCC82950.020.2095289
CCATATG31150.020.1281767
CGGCTTG128800.019.8305139
TGAGGGT579400.019.726492
GGCTTGG129650.019.68353510
AGAAACC71550.019.601458
Produced by FastQC (version 0.11.5)