FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15195756
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC415600.021.43577410
CTGGCGA417750.021.3359839
TCTGGCG429750.021.2444488
GTCTGGC410800.021.2363957
GGCGACC99050.021.03336511
GAGGGTC418150.020.937253
ATGAGGG435450.020.9281481
GGTCTGG423450.020.5790146
GGCGACG103700.020.53571711
TATGCCC76100.020.4406051
GGTGATC96350.020.184773
AGGGTCT429100.020.1696834
TACGCCC62950.020.09487710
GAAGATG72800.019.9147976
GTACGCC64350.019.7602549
CCATATG24850.019.6977637
TGAGGGT445350.019.5958252
CGGCTTG99100.019.5464979
GAGGTGA182250.019.5089041
GGGTCTG444950.019.4733855
Produced by FastQC (version 0.11.5)