FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15288593
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC420250.021.47875610
CTGGCGA423200.021.3290339
TCTGGCG435650.021.3128478
GTCTGGC417000.021.2267387
ATGAGGG439950.021.0184061
GAGGGTC424400.020.9504073
GGCGACC97550.020.81563811
TATGCCC76350.020.7907921
GGCGACG107150.020.60347711
GGTCTGG430250.020.568066
GGTGATC95350.020.246583
AGGGTCT434750.020.1986264
CCATATG23750.020.0080837
GAAGATG75800.019.9536176
TACGCCC63650.019.94310410
GTACGCC64100.019.7859369
TGAGGGT452550.019.638342
GGCTTGG98600.019.61224610
CGGCTTG98700.019.603529
AGAAACC52650.019.5552238
Produced by FastQC (version 0.11.5)