FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15288593
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG211280.1381945349712691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCCG130500.070.0144049
GGCATTA83250.067.3317649
TTTTCCG114850.067.048129
CGTTCCG80900.066.923769
GGGTATA82000.064.6785359
CATTCCG58450.063.9092649
GGACGTT103150.062.746599
TGTTCCG111100.061.8139849
TATTCCG93450.060.3463559
GCACGTT78000.059.9318249
AGTTCCG90550.059.8385669
GATTCCG99750.057.898329
GTACGTT81000.056.610359
CGACGTT82650.056.4571469
TTACGTT69450.055.622579
GGTCAAT45300.055.349449
CGCATTA35700.055.234349
TAACGTT84150.054.6932569
TTTCTCA52950.054.415579
TCACGTT69450.054.398759
Produced by FastQC (version 0.11.5)