FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18235287
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255230.1399648933411358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCCG153300.070.22169
CGTTCCG101500.067.160839
TTTTCCG141950.066.824629
GGCATTA97250.066.250219
GGGTATA94200.065.64329
CATTCCG73250.063.4727679
TGTTCCG133100.062.072029
TATTCCG111300.061.6673169
GGACGTT123900.061.3644339
GCACGTT100300.059.108649
AGTTCCG110950.058.222929
GTACGTT91200.057.597199
GATTCCG121250.057.237699
CGACGTT94400.056.635199
TCACGTT80000.055.567319
TTTCTCA60900.054.432099
TGGTATA80550.053.868879
CGCATTA46600.052.9868289
TTCTGGA94950.052.8606649
GGTCAAT53650.052.836479
Produced by FastQC (version 0.11.5)