FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24153886
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATG1154800.021.52722410
TTAAGAT1157750.021.476179
CAGCTAG1147700.021.4699342
CTAGTTA1151950.021.3871615
TAGTTAA1154050.021.3506436
GGAAAGC405650.021.300371
GTTAAGA1184900.021.2727648
ACAGCTA1202200.021.229271
AGTTAAG1176650.021.1106437
GCTAGTT1166800.021.053694
AAAGCCA373200.020.9853363
AGCTAGT1176500.020.9539663
GAAAGCC375350.020.8697912
GTGTTCC173650.020.7879946
ATCATAC385900.020.72947
TTTCCTA255200.020.5970556
CTAGGGC264100.020.57758110
TTCCTAG259950.020.5125987
TGAGATC395200.020.5073833
CTGAGAT394550.020.4988042
Produced by FastQC (version 0.11.5)