FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25287454
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATG1210600.021.50264510
CAGCTAG1201400.021.4782962
TTAAGAT1214800.021.4246839
GGAAAGC431150.021.383141
CTAGTTA1206100.021.3770475
TAGTTAA1206050.021.3740146
ACAGCTA1252300.021.2716941
GTTAAGA1243300.021.2520438
AGTTAAG1230500.021.143287
GCTAGTT1220750.021.0574324
AAAGCCA400600.020.9748543
AGCTAGT1232250.020.9421423
GAAAGCC403500.020.8211542
ATCATAC413900.020.764787
CTAGGGC274700.020.69651610
CTGAGAT419600.020.6860712
TTCCTAG270600.020.6849277
GTGTTCC178300.020.6591846
TCATACG422650.020.6289628
TTTCCTA265700.020.5820646
Produced by FastQC (version 0.11.5)