FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25287454
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373060.14752770286799138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCCG273450.068.8317269
TTTTCCG246350.068.589049
GGGGTTA221600.068.118359
GGGTATA146700.067.2655949
CGTTCCG175950.066.614069
TGTTCCG237350.065.3349469
CGGGTTA160350.064.507849
TATTCCG194300.063.1006669
CATTCCG121650.062.7764639
GATTCCG185300.060.8446969
AGTTCCG177100.060.4464159
GAGGTTA142050.059.6248059
AGGGTTA154650.059.2186169
GGCATTA94350.059.00529
TGGTATA116900.057.365859
TGGGTTA112500.056.4363569
GGTCCGT132600.056.1822749
AGGTATA102850.056.1120839
TTTCTCA73000.054.4314169
CAGAGCT103650.054.366979
Produced by FastQC (version 0.11.5)