FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26012045
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG383890.1475816299718073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCCG248650.069.048619
GGGTATA153650.068.150689
GGTTCCG279600.068.0778669
GGGGTTA223600.067.205159
CGTTCCG178500.066.6625449
TGTTCCG240000.064.967859
CGGGTTA162700.062.5053529
TATTCCG203750.062.2808079
GATTCCG190700.061.9075439
CATTCCG126200.061.8266039
AGTTCCG182600.060.115399
GGCATTA91050.059.7903679
GAGGTTA155000.059.4414869
AGGGTTA153050.058.61619
GGTCCGT132200.057.1882179
TGGTATA122600.057.125349
AGGTATA106700.055.7205439
TGGGTTA118500.055.623149
ATGAGCT146800.054.3954739
GTGAGCT167400.054.3529669
Produced by FastQC (version 0.11.5)