FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25559236
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATG1222150.021.53703710
TTAAGAT1225800.021.478299
CAGCTAG1215000.021.4622252
CTAGTTA1219100.021.3686755
TAGTTAA1220200.021.3578666
GTTAAGA1257000.021.2724348
ACAGCTA1264350.021.2627741
GGAAAGC433000.021.253271
AGTTAAG1245700.021.1157537
GCTAGTT1235350.021.0306024
AGCTAGT1243750.020.9787753
AAAGCCA399300.020.9222643
GAAAGCC401150.020.8494532
TTCCTAG264250.020.7532867
ATCATAC420250.020.6577977
TCATACG426200.020.6532788
TGAGATC425200.020.6463953
CTGAGAT425100.020.6200832
CGGGACG143150.020.5957932
GTGTTCC187200.020.5818676
Produced by FastQC (version 0.11.5)