FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25559236
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG343360.1343389137296592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCCG274700.069.6168449
TTTTCCG248700.068.624389
GGGGTTA227150.067.726149
GGGTATA146550.067.10249
CGTTCCG180200.065.679679
TGTTCCG234850.064.456089
CGGGTTA161300.063.6799669
CATTCCG121700.063.1347359
TATTCCG197200.062.991579
GATTCCG190750.062.604039
AGTTCCG185650.061.393789
GAGGTTA150600.061.2343759
GGCATTA90750.058.9575779
AGGGTTA157150.058.4387479
TGGTATA122450.057.2298249
TGGGTTA117000.056.4450269
GGTCCGT133950.055.2352879
AGGTATA106750.054.579579
ATGAGCT143500.053.602779
CAGAGCT102850.053.343629
Produced by FastQC (version 0.11.5)