FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26294531
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATG1270900.021.52775410
CAGCTAG1262850.021.4949132
TTAAGAT1276350.021.4478999
CTAGTTA1269200.021.3682735
TAGTTAA1269650.021.3514276
GTTAAGA1304950.021.3116198
GGAAAGC446200.021.2892151
ACAGCTA1318800.021.2524531
AGTTAAG1293650.021.1372667
GCTAGTT1288650.020.9965824
AGCTAGT1298900.020.9130333
AAAGCCA415550.020.897533
GAAAGCC416750.020.8496282
GTGTTCC190850.020.782516
ATCATAC428250.020.72727
TTCCTAG279300.020.6399067
TCATACG436200.020.6394218
CTGAGAT436450.020.6273042
TTTCCTA274550.020.6083896
CTAGGGC286200.020.57658210
Produced by FastQC (version 0.11.5)