FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26294531
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344900.1311679603640772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCCG250100.069.937459
GGTTCCG277900.069.012059
GGGTATA147000.068.4272469
GGGGTTA231850.067.927639
CGTTCCG182850.066.910599
TGTTCCG243650.064.81239
CGGGTTA168650.064.052689
TATTCCG207850.063.0130849
CATTCCG127300.062.42549
GATTCCG190700.061.9055679
GAGGTTA152150.060.832279
AGTTCCG187450.060.326429
GGCATTA92750.059.792239
AGGGTTA159750.059.108689
TGGTATA120250.058.6285869
AGGTATA112000.056.8004729
GGTCCGT137200.055.4120649
TGGGTTA122800.055.4039969
CAGAGCT105450.054.1224679
GCGAGCT165700.053.8316969
Produced by FastQC (version 0.11.5)