FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24704109
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATG1181650.021.49613810
CAGCTAG1174600.021.4720042
TTAAGAT1184100.021.4655939
TAGTTAA1178600.021.3800536
CTAGTTA1178850.021.3740185
ACAGCTA1221250.021.2864381
GTTAAGA1216800.021.2394528
GGAAAGC421700.021.230011
AGTTAAG1204300.021.0836287
AAAGCCA387300.021.0704773
GCTAGTT1195050.021.0382524
GAAAGCC389500.020.9556672
AGCTAGT1204800.020.9319063
TTCCTAG260950.020.7460027
ATCATAC397900.020.6848287
CTGAGAT401650.020.6616382
GAGATCA403150.020.5858654
TTTCCTA259650.020.574536
CTAGGGC266750.020.56705710
TGAGATC403900.020.5530833
Produced by FastQC (version 0.11.5)