FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24704109
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG355800.14402462359601798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCCG235850.069.4435659
GGTTCCG269050.068.914159
CGTTCCG174500.068.1653449
GGGTATA143700.067.929819
GGGGTTA216250.067.523239
TGTTCCG223200.064.582959
CGGGTTA155250.064.436369
CATTCCG123150.063.425999
GATTCCG183550.062.4891559
TATTCCG189000.062.3960849
GGCATTA88300.060.7853369
AGTTCCG176550.060.4655659
GAGGTTA141850.059.258839
AGGGTTA144400.058.5949559
TGGTATA114050.057.6808749
GGTCCGT126200.056.875669
TGGGTTA116000.056.198359
AGGTATA94050.056.0749869
CAGAGCT97800.055.3153579
GTGAGCT163250.054.145999
Produced by FastQC (version 0.11.5)