FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25437925
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAG1226150.021.5173242
TAAGATG1237650.021.50078210
TTAAGAT1242750.021.4329079
TAGTTAA1233750.021.4002516
CTAGTTA1232200.021.3962555
ACAGCTA1279400.021.280061
GGAAAGC438850.021.2564561
GTTAAGA1272200.021.2497378
AGTTAAG1261200.021.088847
GCTAGTT1249500.021.0537824
AGCTAGT1257900.020.9813923
AAAGCCA401400.020.9360053
GAAAGCC403100.020.8696382
TTCCTAG273350.020.6866877
ATCATAC413550.020.681917
TTTCCTA269550.020.635556
CTAGGGC277900.020.62504610
CTGAGAT420650.020.6086082
TGAGATC421350.020.6052043
CGGGACG144700.020.5228312
Produced by FastQC (version 0.11.5)