FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24573744
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAC41950.019.1747531
TATGCCC58250.019.1174051
ACACCGG137200.018.919871
ACACCCT63900.018.5291611
CTCCATT136050.018.4575887
GCTCCAT136000.018.3753726
AACTCCC67200.018.3233621
ACTGAAC56500.018.2683261
CCTACCA77500.018.2166821
GCAGCTC181100.018.102253
CATTCTA150250.018.03090310
TTGAACG62200.018.0271951
ACGCAGC195250.017.9950161
ATAGACC49650.017.974051
GGGACCT62700.017.9711881
TGAGCCG69500.017.8594721
GACTGCG92800.017.845671
CCCTCCT68950.017.8263841
GACTAAC67700.017.8141961
CTGCGGA69900.017.8044991
Produced by FastQC (version 0.11.5)