FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24573744
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG379330.15436394226292907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATTA216150.073.903919
GCACGTT193450.070.515839
GGACGTT269550.070.3304149
CGCATTA95250.066.878569
GTACGTT194550.066.840629
TCACGTT158100.065.585569
GGTCAAT120750.065.4600149
TAACGTT178250.064.703929
CGACGTT197000.064.2836159
ATACGTT167250.063.5728239
TTACGTT170500.063.0589079
GGGTATA130900.063.0138749
TGCATTA124100.063.0080959
GTCATTA124400.062.6853379
GTTCAAT104550.062.5960589
TGACGTT207500.062.054729
GAACGTT234050.060.8256079
AGACGTT213950.060.481889
CTACGTT145400.060.4124379
CCACGTT154200.060.3545539
Produced by FastQC (version 0.11.5)