FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46729917
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGG245350.019.0943951
TATGCCC111350.019.081
CCCATAC73450.018.6343781
CTCCATT254000.018.5611697
AACTCCC129600.018.4816071
GCTCCAT255450.018.4250356
CCTACCA150750.018.2168621
GGGACCT117500.018.1000731
CATTCTA282550.018.09904710
ACACCCT120900.018.0915761
ACGCAGC372500.018.0911481
CCCTCCT128400.018.0802231
CAATGGA140650.018.0658998
TGAGCCG136800.018.0075361
ACCCACT132900.017.9399091
GCAGCTC345100.017.907383
GACTAAC133400.017.8974111
ATAGACC91650.017.8510931
CTGCGGA131050.017.8489461
ACTGAAC114000.017.8450761
Produced by FastQC (version 0.11.5)