FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48097518
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCC116100.019.0323981
ACACCGG253300.018.7546461
AACTCCC133650.018.6327781
ACACCCT127600.018.4899671
CTCCATT263700.018.419887
CCCATAC79400.018.3497221
GCTCCAT262000.018.341936
CCTACCA150150.018.2122291
GGGACCT120450.018.2080121
ACGCAGC382450.018.0263751
CTGCGGA137500.018.0230271
ATAGACC93000.018.009171
CATTCTA290650.017.99103710
TCCCGAT149900.017.9636421
GGGAATG123200.017.9355641
GCAGCTC357450.017.9211623
ACTGAAC118200.017.8656791
CAATGGA142850.017.8407548
AGAAACC95350.017.8112348
CCCTCCT137350.017.8023531
Produced by FastQC (version 0.11.5)