FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77140224
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCC184700.018.9517771
CCCATAC126600.018.774711
ACACCGG409350.018.7285541
GGGACCT191200.018.6010151
CTCCATT421850.018.551727
AACTCCC221800.018.485641
ACACCCT204850.018.4842531
GCTCCAT422150.018.4785616
CCTACCA250500.018.217111
ACGCAGC614350.018.1051671
TCCCGAT240500.018.0915071
GACTAAC218350.018.0721681
CATTCTA470900.017.9530610
GCAGCTC573200.017.9429173
ACTGAAC186650.017.9401931
AGCTCCA450900.017.9373935
ATAGACC156400.017.892161
CCCTCCT217900.017.8721371
CTGCGGA224300.017.8429721
ACCCACT218700.017.831921
Produced by FastQC (version 0.11.5)