FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37383842
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGATC580350.021.1836196
CAAATGC616550.020.84612310
CAGAAGA580300.020.812044
CCTTCCA628600.020.6127455
TTCTCCT679050.020.5865351
TTCCAAA643550.020.4124937
AGAAGAT595250.020.3928155
TCCAAAT633250.020.3693248
CCAAATG635200.020.261779
CTCCTTC667250.020.1906433
CATCAGA674700.020.0932331
AAGATCG605000.020.0768977
TCCTTCC641350.020.0451814
TCTCCTT641200.019.9336222
ATCAGAA614750.019.9127482
GATCGGG621650.019.894819
AGATCGG624400.019.833618
CTTCCAA653350.019.7123666
TTGGAAC182300.019.6338041
CCCATGC106950.019.633156
Produced by FastQC (version 0.11.5)