FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37383842
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG640580.17135210447337115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATTA195350.065.585829
GCACGTT219300.065.302139
GGACGTT304550.062.6643529
TCCCGTG175050.062.2917679
CGACGTT207600.061.3882459
TTCCGTG348700.060.6285559
TCACGTT184450.060.361329
GGTTCCG213150.059.411029
GTACGTT222700.058.7905589
GGGTATA156200.058.7636539
AGACGTT225850.056.277199
CGTTCCG144250.055.9429369
CCACGTT174000.055.511929
TAACGTT225350.054.214619
TGACGTT231850.054.1059889
CACCGTG186800.054.0739869
CTACGTT166850.054.0449689
GAACGTT272300.054.027589
TTACGTT208550.053.3655979
GGTCAAT113000.053.325379
Produced by FastQC (version 0.11.5)