FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19424641
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGATC298250.021.1834956
CAGAAGA296450.020.9485054
CAAATGC329500.020.83684510
CCTTCCA333650.020.7161315
TTCTCCT364100.020.621561
TTCCAAA338700.020.582627
AGAAGAT303450.020.5341345
TCCAAAT338200.020.33018
CCAAATG339750.020.2244019
CTCCTTC355850.020.2001483
TCCTTCC342050.020.0562524
CATCAGA344600.020.0392721
AAGATCG311350.019.9848527
ATCAGAA314750.019.954752
TCTCCTT343800.019.9515272
AGATCGG320800.019.8967368
CTTCCAA346200.019.8094796
GATCGGG323050.019.7513489
TTGGAAC94000.019.55351
ACACCCT76800.019.4640921
Produced by FastQC (version 0.11.5)