FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14900237
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGATC213800.021.1656116
CAAATGC256500.021.00417510
CAGAAGA213600.020.8301074
CCTTCCA261800.020.675595
TTCTCCT285150.020.6617741
TCCAAAT262900.020.5137738
AGAAGAT217500.020.512235
TTCCAAA268250.020.424487
CCAAATG264650.020.3531869
CTCCTTC279600.020.2529013
TCTCCTT268300.020.019442
TCCTTCC269900.019.9654334
CATCAGA250800.019.9257951
AAGATCG224850.019.8955297
CTTCCAA272000.019.7910696
ATCAGAA227900.019.735982
AAATGCT78500.019.6610811
AGATCGG233400.019.6049928
TTGGAAC69450.019.5770231
ACACCCT57700.019.560141
Produced by FastQC (version 0.11.5)