FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14900237
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314340.21096308736565733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGTT86750.065.641869
GGCATTA77700.064.755449
GGACGTT119150.062.4507569
TCCCGTG67600.062.2977759
GTACGTT89150.061.729689
TCACGTT71150.061.3396269
TTCCGTG136600.061.161549
CGACGTT83450.061.1083459
GGTTCCG84000.059.5446479
GGGTATA67700.057.121339
AGACGTT83800.056.999089
CTACGTT63950.056.1515469
CCACGTT66750.055.1330769
TGACGTT92550.054.778439
TTACGTT83750.054.5975349
TAACGTT89300.054.4878469
GAACGTT111050.054.1481329
CACCGTG74200.053.8928649
CGTTCCG57000.053.60439
TGCATTA47050.053.4696929
Produced by FastQC (version 0.11.5)