FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17108328
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGATC228200.021.242016
CAAATGC301950.020.96050610
CAGAAGA228600.020.81514
CCTTCCA307750.020.70855
TTCTCCT336000.020.5519391
TTCCAAA315400.020.4956157
TCCAAAT311500.020.3849878
AGAAGAT234400.020.2859735
CCAAATG312200.020.2793889
CTCCTTC326750.020.2383043
ACACCCT79350.020.1136671
TCCTTCC316150.020.0051984
CTTCCAA318500.019.874866
AGATCGG246400.019.8559678
AAGATCG241100.019.8407977
CATCAGA271150.019.8164421
TCTCCTT318850.019.8117662
GATCGGG248250.019.668129
ATCAGAA245500.019.6601072
TTGGAAC87600.019.4750441
Produced by FastQC (version 0.11.5)