FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17108328
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG355980.20807410285797653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATTA86200.065.320899
GCACGTT96800.065.235949
GGACGTT138550.063.1222349
CGACGTT98050.062.1505629
TTCCGTG156400.061.05349
TCACGTT81000.060.8578389
GGGTATA75750.060.756029
TCCCGTG75350.060.6837659
GTACGTT99050.059.8498769
GGTTCCG94100.058.7983139
AGACGTT98600.056.114829
CGTTCCG61850.055.5156179
TGACGTT105850.054.7203039
TACCGTG145250.054.3006869
GAACGTT123500.054.2635549
CTACGTT74750.054.121439
CCACGTT80350.053.945829
GGTCAAT51150.053.088299
TTACGTT90500.052.497279
CGCATTA46600.052.43559
Produced by FastQC (version 0.11.5)