FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38485969
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGATC598500.021.215656
CAGAAGA601650.020.8522854
CAAATGC643050.020.77385110
CCTTCCA652900.020.5969055
TTCTCCT713750.020.513461
AGAAGAT613000.020.4823445
TTCCAAA669100.020.3891877
CCAAATG663050.020.1737799
CTCCTTC697700.020.1655273
TCCAAAT664400.020.1443758
CATCAGA696400.020.1131671
AAGATCG625350.020.1042217
TCCTTCC670550.019.9054894
AGATCGG648550.019.9006338
TCTCCTT673150.019.8290422
ATCAGAA641400.019.7885062
GATCGGG653250.019.7389289
CTTCCAA679300.019.7009016
CCCATGC109950.019.5976246
TTGGAAC186700.019.4833771
Produced by FastQC (version 0.11.5)