FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33150855
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG666660.20109888568484885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGTT188650.065.302879
GGCATTA177700.064.352759
GGACGTT271700.063.125149
TCCCGTG155500.062.9094359
TCACGTT159500.061.4649859
TTCCGTG302300.061.2899449
CGACGTT181050.060.838349
GTACGTT199400.060.4396489
GGTTCCG185800.059.214389
GGGTATA149550.057.683359
AGACGTT185500.057.0880249
TGACGTT204500.055.8361179
CCACGTT149850.055.781289
CGTTCCG129600.054.856979
CTACGTT147050.054.531549
TAACGTT202500.054.352019
GAACGTT233150.053.841359
CGCATTA86150.053.6185539
TACCGTG273550.053.51729
GGTCAAT101400.053.4334379
Produced by FastQC (version 0.11.5)