FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90131884
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGG3465900.021.6385151
GCGTGGT3350100.021.5121352
TCATACG3410550.021.2717788
GTGGTCA3387750.021.2230644
CATCCAC2814400.021.1846711
AACGTTG1886800.021.1633971
CATACGG3433550.021.1244839
CCATGCT1856400.021.107399
GTTGTCC1752700.021.0918394
GTGAAAG1454450.021.0757968
ATACGGT3439400.021.0620110
TCCATGC1866550.020.9949688
CGGACTG1026200.020.994881
GTCATAC3412900.020.9803187
TTGTCCA1767900.020.9231645
GCTAAGA1391050.020.7539739
CGTGGTC3477800.020.7279323
CGTGAAA1445350.020.7214987
GTCGGAC931350.020.6793797
ACGTGAA1444400.020.6613256
Produced by FastQC (version 0.11.5)