FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91515860
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGG3515850.021.636611
GCGTGGT3397100.021.505942
TCATACG3453600.021.2730718
GTGGTCA3434350.021.2208274
CATCCAC2828850.021.2069441
AACGTTG1900950.021.1800651
CATACGG3473900.021.1446469
CCATGCT1867050.021.1159469
GTGAAAG1471100.021.0949768
GTTGTCC1769150.021.0583724
ATACGGT3485800.021.0472210
CGGACTG1036950.020.9908851
TCCATGC1879400.020.9760178
GTCATAC3466250.020.9343037
TTGTCCA1787700.020.8712125
CGTGAAA1458400.020.7666177
GTCGGAC938950.020.7093077
ACGTGAA1457900.020.7077716
CGTGGTC3531650.020.6962913
GCTAAGA1397800.020.6920499
Produced by FastQC (version 0.11.5)