FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43589115
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAG501650.020.8907092
ACTGCTC629950.020.8122161
AGTCTAG556100.020.505297
TCTAGCC519250.020.4840419
CTAAACA589300.020.34677510
CTAGCCG522750.020.34058210
GTCTAGC547050.020.3277768
GCTAAAC590600.020.2982659
TGAGTCT523100.020.237025
ACACTGA598750.020.1743131
GCTCAGC582650.020.0339054
CCTTTAG249550.020.022972
GAGTCTA535850.019.9810496
TGCTCAG595400.019.9575273
CTGCTCA597050.019.8932762
CTGAGTC530550.019.7185974
CCCGAAA189250.019.63312510
AAGAATG147000.019.5892587
TTGGGAC269250.019.4004659
CAGCCCG156400.019.3824277
Produced by FastQC (version 0.11.5)