FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46913069
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAG530150.021.0350722
ACTGCTC665600.020.8432221
AGTCTAG597400.020.5348287
TCTAGCC555800.020.4885569
CTAAACA620250.020.43955210
GCTAAAC620550.020.4263219
GTCTAGC586650.020.2829728
CTAGCCG562900.020.21430210
TGAGTCT561950.020.1789385
ACACTGA637850.020.1227341
GCTCAGC615650.020.0160964
CCTTTAG264100.020.0085492
GAGTCTA574800.019.8808966
TGCTCAG630050.019.8584333
CTGCTCA631300.019.8209612
AAGAATG157450.019.8050087
CTGAGTC567300.019.7598194
CCCGAAA199550.019.67777610
TTGGGAC282600.019.4413789
TTAGTTG272900.019.3108795
Produced by FastQC (version 0.11.5)