FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46913069
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG726260.15480973969108694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA271650.068.0386669
GGTTCCG380650.067.33489
TTTTCCG329750.066.477199
CGTTCCG249750.063.47169
TGTTCCG320400.061.7584729
CATTCCG170250.059.3609479
GGGGTTA270400.058.781549
TATTCCG272250.057.3830579
GGCATTA158800.055.5052879
AGGTATA188400.055.356649
TGGTATA221500.055.008539
GATTCCG273350.054.991069
CGGGTTA193800.054.6913649
AGTTCCG268850.054.615279
GCGTATA171450.052.227979
GCACGTT172950.051.7258459
GAGGTTA183950.051.058569
GAGTATA150550.049.6830189
GTTTCCG382000.049.6523259
GTGTATA191950.048.8862849
Produced by FastQC (version 0.11.5)