FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36315540
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG567360.15623063845395113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA204200.067.639759
TTTTCCG255000.066.114449
GGTTCCG294050.065.991659
CGTTCCG189750.065.399649
TGTTCCG255050.062.1023989
GGGGTTA210050.059.3219769
CATTCCG136150.058.3087239
TATTCCG212650.057.6173869
CGGGTTA146950.056.1056339
GGCATTA119950.055.554619
TGGTATA168250.055.0144929
AGGTATA143350.054.994669
GATTCCG214800.054.686249
AGTTCCG206100.054.087219
GCACGTT136550.052.8469969
GAGTATA112000.050.770619
GCGTATA136950.050.7376149
GAGGTTA143700.050.5724149
GTTTCCG294600.049.71159
GTGTATA141400.049.261079
Produced by FastQC (version 0.11.5)