FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39110172
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAG447000.021.0072022
ACTGCTC557950.020.8368241
AGTCTAG497800.020.6241137
TCTAGCC462700.020.5554629
CTAGCCG465900.020.39297910
CTAAACA521900.020.33762210
GCTAAAC521950.020.3315129
ACACTGA530050.020.2951241
GTCTAGC489350.020.2654198
CCTTTAG231200.020.2052332
TGAGTCT468500.020.1936115
GAGTCTA480450.020.0484436
GCTCAGC519500.019.9028994
TGCTCAG529050.019.9002633
CTGCTCA530500.019.8248622
CTGAGTC474450.019.7502334
CCCGAAA167100.019.701110
TTGGGAC245550.019.6467179
AAGAATG135700.019.5424827
AGCCCGA170750.019.2993378
Produced by FastQC (version 0.11.5)