FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39110172
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG615440.15736059662432575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCCG321450.067.519439
GGGTATA231650.067.220159
TTTTCCG275650.066.064669
CGTTCCG202400.065.0499959
TGTTCCG272650.060.9307489
GGGGTTA225950.058.2512479
CATTCCG144450.058.0772369
TATTCCG232200.056.8480539
GGCATTA129050.056.0502979
TGGTATA185000.055.5470439
AGGTATA165400.054.857859
CGGGTTA163250.054.799339
GATTCCG229450.054.454739
AGTTCCG214050.053.74649
GCACGTT143800.050.7739149
GCGTATA146850.050.5007559
GAGGTTA147950.050.4699869
GTTTCCG324550.050.388319
GAGTATA131500.049.9643339
GTGTATA157600.049.3212249
Produced by FastQC (version 0.11.5)