FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84464996
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAG931300.020.939782
ACTGCTC1214100.020.8408991
TCTAGCC973300.020.524849
AGTCTAG1047050.020.4700097
CTAAACA1133400.020.39236610
GCTAAAC1137750.020.3095669
CTAGCCG983800.020.29012510
GTCTAGC1030750.020.2302828
TGAGTCT985750.020.0912825
ACACTGA1122700.020.0788231
CCTTTAG490850.020.0575372
GCTCAGC1123500.020.0422864
GAGTCTA1010350.019.88796
CTGCTCA1151300.019.8803392
TGCTCAG1151800.019.8788953
CTGAGTC996850.019.6601164
AAGAATG287800.019.6289487
CCCGAAA360550.019.51217810
TTGGGAC527550.019.4777629
CAGCCCG303100.019.342127
Produced by FastQC (version 0.11.5)