FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31685814
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG467890.14766545053884367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA259850.070.737359
GGACGTT298600.069.883899
GCACGTT217950.067.8395549
GGCATTA163600.066.035539
CGACGTT209850.065.7797859
GTACGTT224300.064.043179
TCACGTT182350.062.9978379
TTACGTT195100.061.7998549
TGGTATA198850.061.1046079
ATACGTT195700.060.9588789
AGACGTT230450.060.932469
GGTCCGT176150.060.848219
TAACGTT214250.060.8384749
AGGTATA170550.059.3823979
ACACGTT193250.059.3565679
CTACGTT165350.058.2169469
TTTCTCA118400.058.1498649
GAACGTT271650.057.495399
CCACGTT178700.057.149789
TGACGTT237300.057.0960359
Produced by FastQC (version 0.11.5)